| 1. | The signal was intensified by increasing the number of fluorophores labeled on the target dna segment
两种引物分别命名为：多标记线性引物和多标记分支引物。 |
| 2. | After getting the cdna sequence of curcin , four fragments of the gene were cloned through pcr and high - level expression in e . coli was achieved . the four target dna fragments , i . e
在得到麻疯树毒蛋白cdna序列后，通过pcr对毒蛋白基因进行了克隆，并实现了部分基因片段在大肠杆菌中的高效表达。 |
| 3. | A chip based on electroelution principle was presented for the recovery of dna fragments , which can make the isolation and collection of target dna fragments possible in a single process by switching electrodes
提出了一种基于电洗脱原理的核酸纯化回收芯片，通过对芯片上电极进行适当的切换操作，可一次完成核酸样品分离和纯化回收。 |
| 4. | A new resolution vector based on tnpi - mediated site - specific recombination system of b . thuringiensis transposon tn4430 was developed . the crylac10 or other target dna , and the gene ori1030 , from a plasmid of wide type b . thitringiensis subsp
利用转座因子构建解离载体的可行性利用苏云金芽胞杆菌转座子tn4430的解离区构建了解离载体。 |
| 5. | The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr , and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5为标签，用锚定pcr法克隆出突变株nm4的tn5侧翼序列。并以此侧翼序列为标签继续用锚定pcr法向两边延伸，克隆出一个5045bp的dna片段。 |
| 6. | Biotin - avitin system ( bas ) was used to immobilize dna on the pdms channel compared to the direct immobilization methds . the hybridization reaction also has been examined between immobilized probe and target dna
针对pdms新型材料，试验了两种dna分子的固定方法，确定了生物素?亲和素介导的dna分子固定化方法，并使用此芯片和固定方法进行了芯片上的dna杂交反应。 |
| 7. | 2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
将自puc18 h d - 3回收的目的片段连同表达运载蛋白dhfr的基因片断一起连接于原核高效表达载体pqe - 80l ，经酶切鉴定，得到重组原核表达载体pqe - 80l dhfr h d - 3 。 |
| 8. | Section - i : to research and establish a kind of high density fermentation technology pattern . what the question that people are concerned about most chiefly was plasmid stability keeps , the high efficiency of target dna is expressed and the influence to the purification in lower reaches
研究并建立一种适于目的蛋白高效表达的高密度发酵工艺模式高密度发酵中，人们最关心的问题主要是质粒稳定性的维持、外源基因的高效表达及对下游纯化的影响。 |
| 9. | Addition of iptg to growing culture of the lysogen induces t7 rna polymerase , which in turn transcribe the target dna in the plasmid . in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg , a 52kd protein with tryptopanase activity was expressed
摸索发酵条件，如改变培养温度和iptg浓度等，发现在30培养条件下， 0 . 2mmiptg诱导时，发酵液中的吲哚含量最高，表明低浓度的诱导剂或低温诱导有利于表达出有活性的色氨酸酶。 |
| 10. | Monofunctional alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng ) is a widely spread environmental mutagen and carcinogen that targets dna and proteins to generate adducts . among the adducts , o6 - alkyl guanine is the predominant mutagenic lesion because of its mispairing properties , which can eventually lead to chromosomal aberrations , point mutations , and cell death . this lesion also appears to be involved in tumor initiation , particularly in gastric carcinogenesis
单功能烷化剂n -甲基- n -硝基- n -亚硝基胍( mnng )是一种在环境中广泛存在的化学诱变剂和致癌剂，它能和dna及蛋白质等生物大分子形成加合物( adduct ) ，其引起的与突变有关的主要dna损伤类型是o ~ 6 -甲基鸟嘌呤，这种损伤与肿瘤尤其是胃癌的发生密切相关。 |
